Generating high-quality libraries for DIA datasets

Lecture for the 2022 EMBO Course on Targeted Proteomics, entitled Generating high-quality predicted libraries for DIA dataset peptide detection

The following topics were discussed:

  • Introduction on library-free, DDA library, predicted library, and chromatogram library approaches
  • Practical considerations when generating predicted libraries
  • Benchmarking results of using predicted libraries
  • Future perspectives